UJI AKTIVITAS ANTI INFLAMASI EKSTRAK KULIT PISANG MULI (MUSA ACUMINATA COLLA) MENGGUNAKAN NADES KOLIN KLORIDA- ASAM LAKTAT
DOI:
https://doi.org/10.51878/healthy.v5i4.15958Keywords:
Anti-inflamasi, Kulit Pisang Muli, NADES, kolin klorida–asam laktat, Musa acuminata Colla.Abstract
ABSTRACT
Long-term use of nonsteroidal anti-inflammatory drugs (NSAIDs) may cause gastrointestinal adverse effects and renal dysfunction, highlighting the need for alternative anti-inflammatory agents derived from natural sources. Muli banana (Musa acuminata Colla) peel contains bioactive compounds with potential anti-inflammatory properties. This study aimed to evaluate the anti-inflammatory activity of Muli banana peel extract obtained using a natural deep eutectic solvent (NADES) composed of choline chloride and lactic acid in male mice. This experimental laboratory study employed ultrasound-assisted extraction using 0.5 g (F1), 1 g (F2), and 1.5 g (F3) of powdered plant material in 10 mL of NADES. The chemical profile was analyzed using liquid chromatography–mass spectrometry (LC–MS), while total phenolic content was determined using the Folin–Ciocalteu method. Anti-inflammatory activity was evaluated by inducing paw edema through intraplantar injection of 1% carrageenan, followed by topical application of the extract directly to the induced paw according to the assigned treatment group. The evaluated parameters included edema volume, edema diameter, and differential leukocyte counts. Data were analyzed using one-way analysis of variance (ANOVA), followed by Tukey's post hoc test (p < 0.05). The results revealed six putatively identified compounds and an increase in total phenolic content across F1–F3. All three formulations significantly reduced edema. F3 exhibited the highest anti-inflammatory activity, with inhibition values of 89.95% based on edema volume and 55.83% based on edema diameter, showing no statistically significant difference compared with the positive control. In conclusion, F3 demonstrated the greatest anti-inflammatory potential among the tested formulations.
ABSTRAK
Penggunaan obat antiinflamasi nonsteroid jangka panjang dapat menimbulkan efek samping gastrointestinal dan gangguan fungsi ginjal, sehingga diperlukan alternatif antiinflamasi berbasis bahan alam. Kulit buah pisang muli (Musa acuminata Colla) mengandung senyawa bioaktif yang berpotensi sebagai antiinflamasi. Penelitian ini bertujuan mengevaluasi aktivitas antiinflamasi ekstrak kulit buah pisang muli menggunakan Natural Deep Eutectic Solvent (NADES) kolin klorida–asam laktat pada mencit putih jantan. Penelitian eksperimental laboratorium dilakukan dengan ekstraksi ultrasound-assisted extraction menggunakan 0,5 g (F1), 1 g (F2), dan 1,5 g (F3) serbuk simplisia dalam 10 mL NADES. Profil senyawa dianalisis menggunakan LC–MS, sedangkan kadar fenolik total ditetapkan dengan metode Folin–Ciocalteu. Uji aktivitas antiinflamasi dilakukan dengan induksi karagenan 1% secara intraplantar pada telapak kaki mencit, kemudian sediaan ekstrak diberikan secara topikal dengan cara dioleskan langsung pada area yang diinduksi sesuai kelompok perlakuan. Parameter pengamatan meliputi volume edema, diameter edema, dan diferensial leukosit. Data dianalisis menggunakan ANOVA satu arah dan uji Tukey (p < 0,05). Hasil menunjukkan enam kandidat senyawa teridentifikasi dan kadar fenolik total meningkat pada F1–F3. Ketiga formula menurunkan edema secara bermakna. F3 menunjukkan aktivitas tertinggi, yaitu 89,95% berdasarkan volume dan 55,83% berdasarkan diameter edema, serta tidak berbeda bermakna dengan kontrol positif. Dengan demikian, F3 menunjukkan potensi antiinflamasi paling tinggi.
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