EFFECT OF MURRAYA PANICULATA LEAF EXTRACT ON HEPATIC INTERLEUKIN-6 EXPRESSION IN PLASMODIUM BERGHEI-INFECTED BALB/c MICE
DOI:
https://doi.org/10.51878/healthy.v5i4.16052Keywords:
Immunohistochemistry, Interleukin-6, Malaria, Murraya paniculata, Plasmodium bergheiAbstract
ABSTRACT
Malaria induces systemic inflammation that may involve hepatic tissue, with interleukin-6 (IL-6) serving as an important inflammatory marker. Murraya paniculata contains bioactive compounds with potential antioxidant and anti-inflammatory activities. This study aimed to evaluate the effect of M. paniculata leaf extract on hepatic IL-6 expression in Plasmodium berghei-infected BALB/c mice. A post-test-only experimental study was conducted using male BALB/c mice (n = 5 per group) divided into six groups: healthy control, infection control, hydroxychloroquine control, and M. paniculata extract groups receiving 50, 100, and 200 mg/kg body weight. Malaria was induced using P. berghei ANKA. One mouse in each group died during the experiment, so 24 mice (n = 4 per group) were analyzed. Hepatic IL-6 expression was evaluated by immunohistochemistry and quantified using ImageJ with the IHC Profiler plugin. Animal-level analysis showed no significant difference among groups (one-way ANOVA, F(5,18) = 1.974, p = 0.132), and none of the extract groups differed significantly from the infection control group in pairwise analysis. Therefore, Murraya paniculata leaf extract did not demonstrate a statistically significant reduction in hepatic IL-6 expression compared with the infection control group. Further studies using larger sample sizes and additional hepatic inflammatory and injury markers are warranted.
ABSTRAK
Malaria menyebabkan peradangan sistemik yang dapat melibatkan jaringan hati, dengan interleukin-6 (IL-6) sebagai salah satu penanda inflamasi penting. Murraya paniculata mengandung senyawa bioaktif yang berpotensi memiliki aktivitas antioksidan dan antiinflamasi. Penelitian ini bertujuan untuk mengevaluasi pengaruh ekstrak daun M. paniculata terhadap ekspresi IL-6 pada jaringan hati mencit BALB/c yang terinfeksi Plasmodium berghei. Penelitian eksperimental dengan desain post-test-only dilakukan menggunakan mencit BALB/c jantan yang dibagi menjadi enam kelompok (n = 5 per kelompok), yaitu kontrol sehat, kontrol infeksi, kontrol hidroksiklorokuin, serta kelompok ekstrak M. paniculata dengan dosis 50, 100, dan 200 mg/kg berat badan. Malaria diinduksi menggunakan P. berghei galur ANKA. Satu mencit pada setiap kelompok mati selama penelitian sehingga 24 mencit (n = 4 per kelompok) dianalisis. Ekspresi IL-6 pada jaringan hati dievaluasi menggunakan metode imunohistokimia dan dikuantifikasi menggunakan ImageJ dengan plugin IHC Profiler. Analisis pada tingkat hewan tidak menunjukkan perbedaan yang signifikan antarkelompok (ANOVA satu arah, F(5,18) = 1,974; p = 0,132), dan perbandingan berpasangan tidak menunjukkan perbedaan yang signifikan antara kelompok ekstrak dan kontrol infeksi. Dengan demikian, ekstrak daun Murraya paniculata belum menunjukkan penurunan ekspresi IL-6 pada jaringan hati yang signifikan secara statistik dibandingkan dengan kelompok kontrol infeksi. Penelitian lebih lanjut dengan jumlah sampel yang lebih besar serta penambahan penanda inflamasi dan cedera hati diperlukan.
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