PERBANDINGAN HASIL PEMERIKSAAN Salmonella typhi METODE WIDAL SLIDE DENGAN RAPID TEST IMUNOKROMATOGRAFI PADA PASIEN SUSPEK DEMAM TIFOID DI RUMAH SAKIT DKT SIDOARJO
DOI:
https://doi.org/10.51878/cendekia.v6i4.15719Keywords:
Widal slide, Imunokromatografi, Demam TifoidAbstract
Typhoid fever remains a public health concern that requires laboratory testing to support accurate diagnosis. Serological tests, including the Widal slide test and immunochromatographic rapid tests, are used in healthcare services, but they may produce different results. This study aimed to analyze differences between Widal slide and IgM/IgG immunochromatographic rapid test results in patients suspected of having typhoid fever at DKT Hospital, Sidoarjo. An analytical comparative design with a cross-sectional approach was employed. The sample consisted of 25 patients suspected of having typhoid fever, selected through purposive sampling between April and May 2026. Data were obtained from laboratory examination results and analyzed using the Mann–Whitney test. The results showed that the Widal slide test was positive in 5 patients (20%) and negative in 20 patients (80%), whereas the rapid test was positive in 8 patients (32%) and negative in 17 patients (68%). Statistical analysis yielded a significance value of 0.005 (p < 0.05), indicating a statistically significant difference between the results of the two tests. These findings indicate that the two methods produce different result distributions. Test results should be interpreted in conjunction with patients’ clinical conditions and other supporting examinations.
ABSTRAK
Demam tifoid masih menjadi masalah kesehatan masyarakat yang memerlukan pemeriksaan laboratorium untuk mendukung penegakan diagnosis secara tepat. Pemeriksaan serologis, seperti Widal slide dan rapid test imunokromatografi, digunakan dalam pelayanan kesehatan, tetapi keduanya dapat menunjukkan hasil yang berbeda. Penelitian ini bertujuan menganalisis perbedaan hasil pemeriksaan Widal slide dan rapid test imunokromatografi IgM/IgG pada pasien suspek demam tifoid di Rumah Sakit DKT Sidoarjo. Penelitian menggunakan desain komparatif analitik dengan pendekatan cross-sectional. Sampel terdiri atas 25 pasien suspek demam tifoid yang dipilih menggunakan teknik purposive sampling pada April–Mei 2026. Data diperoleh dari hasil pemeriksaan laboratorium dan dianalisis menggunakan uji Mann–Whitney. Hasil menunjukkan bahwa pemeriksaan Widal slide memberikan hasil positif pada 5 pasien (20%) dan negatif pada 20 pasien (80%), sedangkan rapid test menunjukkan hasil positif pada 8 pasien (32%) dan negatif pada 17 pasien (68%). Uji statistik menghasilkan nilai signifikansi 0,005 (p < 0,05), yang menunjukkan adanya perbedaan bermakna antara hasil kedua pemeriksaan. Penelitian ini menunjukkan bahwa kedua metode menghasilkan distribusi hasil yang berbeda. Interpretasi hasil perlu mempertimbangkan kondisi klinis pasien dan pemeriksaan penunjang lainnya.
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